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KMID : 0881720100250030258
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Journal of Food Hygiene and Safety
2010 Volume.25 No. 3 p.258 ~ p.262
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Role of p53-dependent PI3K in Radioresistance of Colon Cancer Cell
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Lee Heui-Kwan
Kim Jong-Suk
Kwon Hyoung-Cheol
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Abstract
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Radiotherapy is one of the major therapies for cancer treatment. p53 acts as a central mediator of the cellular response to stressful stimuli, such as radiation. Recently it has been known that activation of the phosphatidylinositol- 3-kinase (PI3K) pathway is associated with radioresistance. In this study, we investigated whether X-irradiation up-regulates PI3K in a p53-dependent manner in human colon cancer cells. In order to study this phenomenon, we have treated p53-wild type and p53-mutant type HCT116 cells with X-ray. Treatment of wild type HCT116 cells with 8 Gy resulted in a marked increase in PI3K (p85), which paralleled an increase in PTEN, a counterpart of PI3K. However, these effects of X-rays in the p53-mutant cells were not observed. These results suggest that the X-irradiation- induced up-regulation of PI3K/PTEN pathway is p53-dependent.
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KEYWORD
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X-irradiation, p53, PI3K, PTEN, Colon cancer
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